Fig. 8

VWYa-positive neural architecture in a V. multiformis tentacle. a-b’ The main trunk (a, a’) and tentilla (b, b’) were stained with anti-VWYa antibody (green), phalloidin (magenta), and DAPI (blue). Putative sensory cells on the surfaces were positive for VWYa and most of these cells have phalloidin-positive processes in their cell bodies. In the main trunk, neurite-like elongations of putative sensory neurons were positive for VWYa (arrowheads in a and a’). c-c’’ Magnified images of VWYa-positive neurons in the main trunk. VWYa-positive neurons (green) which have phalloidin-positive processes (magenta) partially overlapped with α-tubulin-positive putative sensory cells (yellow). d VWYa-positive putative sensory cells (green) with phalloidin-positive processes (magenta) on the surface of the tentilla. Scale bar, 10 μm