Fig. 4

Neural and muscular organizations of V. multiformis tentacles. The main trunk (a-a”) and tentilla (b-b”) were stained with anti-α-tubulin antibody (green), phalloidin (magenta), and DAPI (blue). a-a” The α-tubulin positive central fiber and putative sensory neurons that extend neurites toward central fibers and bundles of phalloidin-positive thick muscular fibers were observed in the main trunk. b-b” Only the cell bodies of putative sensory cells were positive for α-tubulin; no neurite-like extensions were observed in tentilla. Muscle of the tentilla was a thick, single fiber. Arrowheads indicate phalloidin-positive projections of putative sensory cells on the surfaces. c The schematic drawings of tentacles reconstructed from the immunostaining results. Scale bar, 20 μm