Fig. 6

Instar 2 of Nymphon micronesicum. A Anterior view of adult male bearing > 25 specimens of instar 2; stereomicroscopic autofluorescence image. B Comparison of instar 1 (left bottom corner) and two instar 2 specimens after Sytox staining, epifluorescence image. C-E CLSM scans of instar 2, autofluorescence (magenta) and Sytox staining (gray). C Anterior view (blend mode). The white arrows indicate cheliphoral scape borders. The small arrowheads highlight fibrous secretions from the attachment gland spine. D Lateral view (upper row: blend mode; left bottom corner: virtual sagittal section). The oval and the double arrow highlight the buds of oviger and leg 4, respectively. The small arrows indicate podomere borders along leg 1. The stippled line marks coxae 1–3, between which podomere borders could not be confidently identified. The black arrowheads point to trunk segment borders underneath the dorsal cuticular cover. The star and asterisks highlight internal and externally protruding parts of the ruptured pharynx. E Magnification of posterior body pole (virtual sagittal section). The arrowhead marks the proctodeum at the tip of the anal tubercle. Abbreviations: ac – auxiliary claw; ags – attachment gland spine; at – anal tubercle; br – brain; ch – cheliphore; ey – eye; fe – femur; l1&3 – leg pairs 1 and 3; lg1–4 – leg ganglia 1–4; mc – main claw; mg – midgut; ot – ocular tubercle; pa – palp; pg1&2 – posterior ganglia 1&2; pha – pharynx; pr – proboscis; pro – propodus; sc - scape; seg – subesophageal ganglion; ta – tarsus; tb1&2 – tibia 1&2