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Fig. 4 | Zoological Letters

Fig. 4

From: Epimorphic development in tropical shallow-water Nymphonidae (Arthropoda: Pycnogonida) revealed by fluorescence imaging

Fig. 4

Advanced embryonic stage of Nymphon micronesicum. A Lateral view of adult male bearing embryos and hatched instars; stereomicroscopic autofluorescence image. White arrowheads indicate spherical embryos still contained in the egg membranes. B Embryos after Sytox staining; epifluorescence image. C-E CLSM scans of Sytox-stained embryos (blend mode). Stars mark one side of paired segmental depressions related to nervous system development (“ventral organs”). The ovals indicate the putative primordium of the future oviger. C Ventral view. D Ventrolateral view. E Anterior view. Abbreviations: ch – cheliphore; l1–3 – leg pairs 1–3; ov – oviger; pa – palp; pchl – pre-cheliphoral lobe; pr – proboscis

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