Fig. 3

Instar 2 of Nymphon floridanum. Comparison of SEM and CLSM autofluorescence imaging. A Lateral view; SEM. The surface of the entire instar is significantly collapsed. A’ Lateral view; CLSM. Gradual rehydration into distilled water has reversed the ethanol-induced structural collapse. The double arrow marks the small limb bud of leg 4. B Anterolateral view of the anterior body region; SEM. The cuticular collapse hampers reliable assessment of cheliphore structure, proboscis shape and the presence of the ocular tubercle. B’ Anterolateral view of anterior body region; CLSM. The cheliphore’s scape borders (arrows) and moveable finger (asterisk) are recognizable. The ocular tubercle and barrel-shaped proboscis can be readily discerned. The arrowhead points to an indistinct division in the tarsus–propodus precursor. The stippled line indicates the leg region comprising coxae 1–3. C Detail of the cheliphores; CLSM. White arrows highlight the scape borders. Small arrowheads mark fibrous secretions emanating from the attachment gland spine. D Detail of the distal podomeres of legs 1–3; CLSM. Large arrowheads mark a notch near the proximal margin of the tarsus-propodus precursor of legs 1 and 2. Small arrowheads point to setae. The double arrow highlights the tip of leg 3 that still lacks the main and auxiliary claws. Abbreviations: ac – auxiliary claw; ags – attachment gland spine; ch – cheliphore; l1–3 – leg pairs 1–3; mc – main claw; pa – palp; ot – ocular tubercle; ov – oviger; pr – proboscis; sc – scape; ta + pro – tarsus–propodus precursor; ta + pro + mc – tarsus-propodus–main claw precursor; tb1 – tibia 1; tb2 – tibia 2