Fig. 2

Hatching instar 1 of Nymphon floridanum. Comparison of SEM and CLSM imaging. A Lateral view; SEM. All appendages are collapsed and difficult to distinguish. The small arrowhead marks a fibrous strand emanating from the cheliphoral attachment gland spine. B Lateral view; CLSM; autofluorescence (magenta) and nuclear staining (gray) shown as single channels (blend mode) and combined (maximum intensity projection). The yolk-filled proximal portions of leg pairs 1–3 are coiled beneath the cuticle (arrowheads). The green stippled line exemplarily outlines leg 1. The double arrow marks the tiny bud of leg 4. C Ventral view; autofluorescence and nuclear staining (maximum intensity projection, same colors as in B). The folded leg pairs 1–3 cover the ventral side of the trunk. Abbreviations: ags – attachment gland spine; ch – cheliphore; l1-3 – leg pairs 1–3; pa – palp; pr – proboscis